Sunlong Biotech Co.,Ltd

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Product Name:	Bovine substance P (SP) ELISA kit
Product Type :	ELISA kit
Code :	CSB-EQ027765BO
Size :	96T,5×96T,10×96T
Abbreviation :	SP
Species :	Bovine
Sample Types :	serum, plasma, tissue homogenates, cell lysates
Detect Range :	0.156 ng/ml-10 ng/ml
Sensitivity :	0.039 ng/ml
Assay Time :	1-3h
Sample Volume :	50-100ul
Detection Wavelengt :	450nm
Research Area :	Neuroscience
Protocol :
Precision :	Intra-assay Precision (Precision within an assay): CV%<8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV%<10%Three samples of known concentration were tested in twenty assays to assess.
Linearity :	To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.SampleSerum(n=4)1:1Average %94Range %88-971:2Average %100Range %96-1031:4Average %91Range %86-941:8Average %97Range %92-100
Recovery :	The recovery of bovine SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.Sample TypeAverage % RecoveryRangeSerum (n=5)9487-99EDTA plasma (n=4)9287-96
Typical Data :	These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.ng/mlOD1OD2AverageCorrected102.5262.4322.4792.34251.9481.9791.9641.8272.51.2791.2231.2511.1141.250.8020.7850.7940.6570.6250.4900.4720.4810.3440.3120.3420.3370.3400.2030.1560.2420.2380.2400.10300.1380.1360.137